Abstract 42: Human Adipose-Derived Stem Cells with BRCA Mutation Promote Breast Tumor Cell Migration

F ray, M ay 5, 2017 RESULTS: Laser Doppler flowmetry confirmed a reactive hyperemia following tourniquet release. In addition, both the phosphorescence lifetime biosensor and NIR methods suggested that the tissue O 2 content temporarily but significantly exceeded baseline following tourniquet release (p<0.05). We term this phenomenon reactive hyperoxia. This suggests that the O 2 supply transiently exceeds oxygen consumption. Even though tissue O 2 consumption was expected to be elevated following ischemia to repay the O 2 debt developed during occlusion, the elevated O 2 content indicates that the increased consumption is met with an even greater elevated O 2 supply. The NIR spectroscopy and phosphorescence lifetime data are consistent. The phosphorescence lifetime biosensor also demonstrates a more prominent response magnitude in general and a faster response over time (71 secs vs. 143 secs in NIR; p = 0.0053), suggesting that the sensing elements may have gained greater proximity to the blood supply via vascular ingrowth through the porous pHEMA scaffold.